MYC Split CISH Probe, Size:400 uL

Detail

Size:400 uL,
Product Description:MYC Split CISH Probe is designed for the qualitative detection of human MYC gene at 8q24.21 in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).,
Clone Name:NONE,
Isotype:NONE,
Geneid:4609,
Gene Name:MYC,
Gene Alias:bHLHe39|c-Myc,
Gene Description:v-myc myelocytomatosis viral oncogene homolog (avian),
Genbank Accession:NONE,
Immunogen:NONE,
Immunogen Sequence Protein Sequence:NONE,
Protein Accession:NONE,
Supplied Product:Reagent Provided:

1. Digoxigenin-labeled polynucleotides targeting sequences mapping in 8q24.21* (chr8:130,373,051-130,847,951) distal to the MYC breakpoint region
2. Dinitrophenyl-labeled polynucleotides targeting sequences mapping in 8q24.21* (chr8:127,888,765-128,363,281) proximal to the MYC breakpoint region
3. Formamide based hybridization buffer

*according to Human Genome Assembly GRCh37/hg19,
Form:NONE,
Concentration:NONE,
Target Refseq:NONE,
Target Region:NONE,
Plasmid:NONE,
Formulation:NONE,
Lysis Buffer:NONE,
Host Cell:NONE,
Organism:NONE,
Tissue:NONE,
Preparation Method:NONE,
Recommend Dilutions:The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (18-25°C) and mix briefly before use.,
Storage Buffer:NONE,
Storage Instruction:Store at 2-8°C in an upright position. Return to storage conditions immediately after use.,
Quality Control Testing:NONE,
Note:The probe is intended to be used in combination with the CISH Implementation Kit 2 (Catalog #: KA5366), which provides necessary reagents for specimen pretreatment and post-hybridization processing.

Hybridization signals of digoxigenin-labeled polynucleotides appear dark green distinct dot-shaped (distal to the MYC breakpoint region), and dinitrophenyl-labeled polynucleotides appear bright red distinct dot-shaped (proximal to the MYC breakpoint region).
Normal situation: In interphases of normal cells or cells without a translocation involving the MYC gene region, two red/green fusion signals appear.
Aberrant situation: One MYC gene region affected by a translocation is indicated by one separate green signal and one separate red signal. Other signal distribution may be observed in some abnormal samples which might result in a different signal pattern than described above, indicating variant rearrangements.
Unexpected signal patterns should be further investigated.,
Tag:NONE,
Conjugate Tag:NONE,
Type Clonality:DNA/RNA,
Raised In Host Species:NONE,
Antigen Species Target Species:Human,
Specificity:NONE,
Species Reactivity Cross Reactivity:Human,
Application Key:CISH-P,CISH-Ce

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