MYCN CISH Probe, Size:400 uL

Detail

Size:400 uL,
Product Description:MYCN CISH Probe is designed for the qualitative detection human MYCN gene in formalin-fixed, paraffin-embedded tissue or cells by chromogenic in situ hybridization (CISH).,
Clone Name:NONE,
Isotype:NONE,
Geneid:4613,
Gene Name:MYCN,
Gene Alias:MODED|N-myc|NMYC|ODED|bHLHe37,
Gene Description:v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian),
Genbank Accession:NONE,
Immunogen:NONE,
Immunogen Sequence Protein Sequence:NONE,
Protein Accession:NONE,
Supplied Product:Reagent Provided:

Digoxigenin-labeled oligonucleotides targeting sequences mapping in 2p24.3* (chr2:15,846,046-16,213,717) harboring the MYCN gene.
2. Formamide based hybridization buffer.
*According to Human Genome Assembly GRCh37/hg19,
Form:NONE,
Concentration:NONE,
Target Refseq:NONE,
Target Region:NONE,
Plasmid:NONE,
Formulation:NONE,
Lysis Buffer:NONE,
Host Cell:NONE,
Organism:NONE,
Tissue:NONE,
Preparation Method:NONE,
Recommend Dilutions:The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (18-25°C) and mix briefly before use.,
Storage Buffer:NONE,
Storage Instruction:Store at 2-8°C in an upright position. Return to storage conditions immediately after use.,
Quality Control Testing:NONE,
Note:The probe is intended to be used in combination with the CISH Implementation Kit 2 (Catalog #: KA5366), which provides necessary reagents for specimen pretreatment and post-hybridization processing.

Hybridization signals of digoxigenin-labeled polynucleotides appear as brown- to dark brown colored distinct dots (MYCN gene).
Normal situation: In interphases of normal cells or cells without an amplification involving the MYCN gene locus, two distinct dot-shaped brown signals per nucleus appear.
Aberrant situation: In a cell with amplification of the MYCN gene locus or aneuploidy of chromosome 2, multiple copies of the brown signal or brown signal clusters will be observed.,
Tag:NONE,
Conjugate Tag:NONE,
Type Clonality:DNA/RNA,
Raised In Host Species:NONE,
Antigen Species Target Species:Human,
Specificity:NONE,
Species Reactivity Cross Reactivity:Human,
Application Key:CISH-P

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