Nuclear/Cytosolic Fractionation Kit, Size:20 preps

Catalog Number: AKR-171

Size: 20 preps,
Shipping TempBlue Ice,
Product Description: Cell Biolabs’ Nuclear/Cytosolic Fractionation Kit provides a simple and fast tool to isolate nucl...

Detail

Size: 20 preps,
Shipping TempBlue Ice,
Product Description: Cell Biolabs’ Nuclear/Cytosolic Fractionation Kit provides a simple and fast tool to isolate nuclear extract from the cytoplasmic fraction of mammalian cells.  The procedure has been optimized to provide extraction, with high protein recovery and low cross-contamination, in less than 2 hours.  The extracted protein fractions are functional and suitable for downstream assays such as DNA footprinting, RNA splicing, gel shift assays (EMSA), reporter assays, enzyme activity assays, and Western blotting.,
Simple and fast tool to isolate nuclear extract from the cytoplasmic fraction of mammalian cells,
Extraction in less than 2 hours,
Each prep can accommodate up to 5 x 106cells,
,
LMO Content: None,
Host: ,
Clone: ,
Isotype: ,
Storage Temp: Upon receipt, aliquot and store Dithiothreitol and Protease Inhibitor Cocktail at -20ºC and avoid multiple freeze/thaw cycles. Store all other components at 4ºC.,
LICENSE RESTRICTIONS: ,
Dangerous Goods Transportation: ,
Can ship 2-day (within US only): ,
Detection Method: N/A

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