SS18 Split CISH Probe, Size:100 uL

Detail

Size:100 uL,
Product Description:SS18 Split CISH Probe is designed for the qualitative detection of human SS18 gene at 18q11.2 in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).,
Clone Name:NONE,
Isotype:NONE,
Geneid:6760,
Gene Name:SS18,
Gene Alias:MGC116875|SSXT|SYT|SYT-SSX1|SYT-SSX2,
Gene Description:synovial sarcoma translocation, chromosome 18,
Genbank Accession:NONE,
Immunogen:NONE,
Immunogen Sequence Protein Sequence:NONE,
Protein Accession:NONE,
Supplied Product:Reagent Provided:

1. Digoxigenin-labeled polynucleotides targeting sequences mapping in 18q11.2* (chr18:23,109,942-23,262,464) proximal to the SS18 breakpoint region
2. Dinitrophenyl-labeled polynucleotides targeting sequences mapping in 18q11.2* (chr18:23,772,255-24,137,169) distal to the SS18 breakpoint region
3. Formamide based hybridization buffer

*according to Human Genome Assembly GRCh37/hg19,
Form:NONE,
Concentration:NONE,
Target Refseq:NONE,
Target Region:NONE,
Plasmid:NONE,
Formulation:NONE,
Lysis Buffer:NONE,
Host Cell:NONE,
Organism:NONE,
Tissue:NONE,
Preparation Method:NONE,
Recommend Dilutions:The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (18-25°C) and mix briefly before use.,
Storage Buffer:NONE,
Storage Instruction:Store at 2-8°C in an upright position. Return to storage conditions immediately after use.,
Quality Control Testing:NONE,
Note:The probe is intended to be used in combination with the CISH Implementation Kit 2 (Catalog #: KA5366), which provides necessary reagents for specimen pretreatment and post-hybridization processing.

Hybridization signals of digoxigenin-labeled polynucleotides appear dark green distinct dot-shaped (proximal to the SS18 breakpoint region), and dinitrophenyl-labeled polynucleotides appear bright red distinct dot-shaped (distal to the SS18 breakpoint region).
Normal situation: In interphases of normal cells or cells without a translocation involving the SS18 gene region, two red/green fusion signals appear.
Aberrant situation: One SS18 gene region affected by a translocation is indicated by one separate green signal and one separate red signal. Other signal distribution may be observed in some abnormal samples which might result in a different signal pattern than described above, indicating variant rearrangements.
Unexpected signal patterns should be further investigated.,
Tag:NONE,
Conjugate Tag:NONE,
Type Clonality:DNA/RNA,
Raised In Host Species:NONE,
Antigen Species Target Species:Human,
Specificity:NONE,
Species Reactivity Cross Reactivity:Human,
Application Key:CISH-P,CISH-Ce

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