CDKN2A/CEN9 CISH Probe, Size:400 uL

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Size:400 uL,
Product Description:CDKN2A/CEN9 CISH Probe is designed for the qualitative detection of human CDKN2A gene deletions and the detection of chromosome 9 classical satellites III in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).,
Clone Name:NONE,
Isotype:NONE,
Geneid:1029,
Gene Name:CDKN2A,
Gene Alias:ARF|CDK4I|CDKN2|CMM2|INK4|INK4a|MLM|MTS1|TP16|p14|p14ARF|p16|p16INK4|p16INK4a|p19,
Gene Description:cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4),
Genbank Accession:NONE,
Immunogen:NONE,
Immunogen Sequence Protein Sequence:NONE,
Protein Accession:NONE,
Supplied Product:Reagent Provided:

This Probe is composed of:
1. Digoxigenin-labeled polynucleotides, which target sequences mapping in 9p21.3* (chr9:21,742,629-22,056,853) harboring the CDKN2A gene region.
2. Dinitrophenyl-labeled polynucleotides, which target sequences mapping in chromosomal region 9q12 specific for the classical satellite III region D9Z3 of chromosome 9.
3. Formamide based hybridization buffer.

*according to Human Genome Assembly GRCh37/hg19,
Form:NONE,
Concentration:NONE,
Target Refseq:NONE,
Target Region:NONE,
Plasmid:NONE,
Formulation:NONE,
Lysis Buffer:NONE,
Host Cell:NONE,
Organism:NONE,
Tissue:NONE,
Preparation Method:NONE,
Recommend Dilutions:The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (18-25°C) and mix briefly before use.,
Storage Buffer:NONE,
Storage Instruction:Store at 2-8°C in an upright position. Return to storage conditions immediately after use.,
Quality Control Testing:NONE,
Note:The probe is intended to be used in combination with the CISH Implementation Kit 2 (Catalog #: KA5366), which provides necessary reagents for specimen pretreatment and post-hybridization processing.

Interpretation of results:
Using the CISH Implementation Kit 2 (Cat # KA5366), hybridization signals of Digoxigenin-labeled polynucleotides appear as dark green colored distinct dots (CDK4 gene region), and Dinitrophenyl-labeled polynucleotides appear as bright red colored distinct dots (CEN 12).
Normal situation: In interphases of normal cells or cells without an amplification involving the CDK4 gene region, two distinct dot-shaped green and two distinct dot-shaped red signals appear.
Aberrant situation: In cells with an amplification of the CDK4 gene region, an increased number of green signals or green signal clusters will be observed.
Overlapping signals may appear as brown signals. Other signal patterns than those described above may be observed in some abnormal samples. These unexpected signal patterns should be further investigated.,
Tag:NONE,
Conjugate Tag:NONE,
Type Clonality:DNA/RNA,
Raised In Host Species:NONE,
Antigen Species Target Species:Human,
Specificity:NONE,
Species Reactivity Cross Reactivity:Human,
Application Key:CISH-Ce

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