DAG (Diacylglycerol) Assay Kit, Size:100 assays

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Size: 100 assays,
Shipping TempDry Ice,
Product Description: Our DAG (Diacylglycerol) Assay Kit measures diacylglycerol in cell lysate samples by a coupled enzymatic reaction system. First, kinase is used to phosphorylate DAG in samples, yielding phosphatidic acid. Next, lipase is used to hydrolyze phosphatidic acid to glycerol-3-phosphate. The glycerol-3-phosphate product is then oxidized by glycerol-3-phosphate oxidase, producing hydrogen peroxide. The hydrogen peroxide released from this reaction reacts specifically with the kit’s Fluorometric Probe and is detected at ex. 530-560 nm/em. 585-595 nm. DAG levels in unknown samples are determined based on the provided DAG standard curve.,
Suitable for use with cell lysates,
Detection sensitivity of 4uM DAG,
DAG standard included,
NOTE: Each sample replicate requires 2 assays for proper data calculation.,
LMO Content: None,
Host: ,
Clone: ,
Isotype: ,
Storage Temp: Store the entire kit at -80ºC. Avoid multiple freeze/thaws by aliquoting. The Fluorometric Probe is light sensitive and should be maintained in amber tubes. ,
LICENSE RESTRICTIONS: ,
Dangerous Goods Transportation: ,
Can ship 2-day (within US only): ,
Detection Method: Fluorometric

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