EGFR CISH Probe, Size:400 uL

Detay

Size:400 uL,
Product Description:EGFR CISH Probe is designed for the qualitative detection of human EGFR gene in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).,
Clone Name:NONE,
Isotype:NONE,
Geneid:1956,
Gene Name:EGFR,
Gene Alias:ERBB|ERBB1|HER1|PIG61|mENA,
Gene Description:epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian),
Genbank Accession:NONE,
Immunogen:NONE,
Immunogen Sequence Protein Sequence:NONE,
Protein Accession:NONE,
Supplied Product:Reagent Provided:

1. Digoxigenin-labeled polynucleotides targeting sequences mapping in 7p11.2-7p12* (chr7:55,034,991-55,380,617) harboring the EGFR gene
2. Formamide based hybridization buffer

*according to Human Genome Assembly GRCh37/hg19,
Form:NONE,
Concentration:NONE,
Target Refseq:NONE,
Target Region:NONE,
Plasmid:NONE,
Formulation:NONE,
Lysis Buffer:NONE,
Host Cell:NONE,
Organism:NONE,
Tissue:NONE,
Preparation Method:NONE,
Recommend Dilutions:The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (18-25°C) and mix briefly before use.,
Storage Buffer:NONE,
Storage Instruction:Store at 2-8°C in an upright position. Return to storage conditions immediately after use.,
Quality Control Testing:NONE,
Note:The probe is intended to be used in combination with the CISH Implementation Kit 1 (Catalog #: KA5365), which provides necessary reagents for specimen pretreatment and post-hybridization processing.

Hybridization signals of digoxigenin-labeled polynucleotides appear as brown- to dark brown colored distinct dots.
Normal situation: In interphases of normal cells or cells without an amplification involving the 7p11.2 band, two distinct dot-shaped brown signals per nuclei appear.
Aberrant situation: In a cell with amplification of the EGFR gene locus or aneuploidy of chromosome 7, multiple copies of the brown signal or brown signal clusters will be observed. Other signal distribution may be observed in some abnormal samples which might result in a different signal pattern than described above, indicating variant rearrangements.
Unexpected signal patterns should be further investigated.,
Tag:NONE,
Conjugate Tag:NONE,
Type Clonality:DNA/RNA,
Raised In Host Species:NONE,
Antigen Species Target Species:Human,
Specificity:NONE,
Species Reactivity Cross Reactivity:Human,
Application Key:CISH-P,CISH-Ce

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