RET Split CISH Probe, Size:400 uL

Detay

Size:400 uL,
Product Description:RET Split CISH Probe is designed for the qualitative detection of human RET gene at 10q11.21 in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).,
Clone Name:NONE,
Isotype:NONE,
Geneid:5979,
Gene Name:RET,
Gene Alias:CDHF12|HSCR1|MEN2A|MEN2B|MTC1|PTC|RET-ELE1|RET51,
Gene Description:ret proto-oncogene,
Genbank Accession:NONE,
Immunogen:NONE,
Immunogen Sequence Protein Sequence:NONE,
Protein Accession:NONE,
Supplied Product:Reagent Provided:

1. Digoxigenin-labeled polynucleotides targeting sequences mapping in 10q11.21* (chr10:43,687,278-43,856,587) distal to the RET breakpoint region
2. Dinitrophenyl-labeled polynucleotides targeting sequences mapping in 10q11.21* (chr10:43,340,888-43,510,171) proximal to the RET breakpoint region
3. Formamide based hybridization buffer

*according to Human Genome Assembly GRCh37/hg19,
Form:NONE,
Concentration:NONE,
Target Refseq:NONE,
Target Region:NONE,
Plasmid:NONE,
Formulation:NONE,
Lysis Buffer:NONE,
Host Cell:NONE,
Organism:NONE,
Tissue:NONE,
Preparation Method:NONE,
Recommend Dilutions:The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (18-25°C) and mix briefly before use.,
Storage Buffer:NONE,
Storage Instruction:Store at 2-8°C in an upright position. Return to storage conditions immediately after use.,
Quality Control Testing:NONE,
Note:The probe is intended to be used in combination with the CISH Implementation Kit 2 (Catalog #: KA5366), which provides necessary reagents for specimen pretreatment and post-hybridization processing.

Hybridization signals of digoxigenin-labeled polynucleotides appear dark green colored distinct dotshaped (distal to the RET breakpoint region), and dinitrophenyl-labeled polynucleotides appear bright red distinct dot-shaped (proximal to the RET breakpoint region)).
Normal situation: In interphases of normal cells or cells without a translocation involving the RET gene region, two red/green fusion signals appear.
Aberrant situation: One RET gene region affected by a translocation is indicated by one separate green signal and one separate red signal. Isolated green signals are the result of deletions proximal to the RET breakpoint region. Other signal distribution may be observed in some abnormal samples which might result in a different signal pattern than described above, indicating variant rearrangements.
Unexpected signal patterns should be further investigated.,
Tag:NONE,
Conjugate Tag:NONE,
Type Clonality:DNA/RNA,
Raised In Host Species:NONE,
Antigen Species Target Species:Human,
Specificity:NONE,
Species Reactivity Cross Reactivity:Human,
Application Key:CISH-Ce

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