S-Adenosylmethionine (SAM) and S-Adenosylhomocysteine (SAH) ELISA Combo Kit, Size:96 assays

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Size: 96 assays,
Shipping Temp2 parts: 1 RT / 1 Dry Ice,
Product Description: S-adenosylmethionine (SAM) is a substrate for methyl transfers to various biomolecules that yields S-adenosylhomocysteine (SAH). SAH is then hydrolyzed to homocysteine and adenosine, which is converted to methionine, and ultimately back to SAM. SAH can act as a methylation inhibitor, therefore the SAM/SAH ratio, or methylation potential, is important for maintaining proper methylation in the cell. Our S-Adenosylmethionine (SAM) and S-Adenosylhomocysteine (SAH) ELISA Combo Kit is designed for detection and quantitation of both SAM and SAH in plasma, serum, lysates, or biological fluid samples. This assay is a competitive ELISA where samples and an anti-SAM or anti-SAH antibody are added to a plate coated with a SAM or SAH conjugate. The sample and conjugate compete for antibody binding, which generates a reverse curve. Samples with high SAM and SAH levels will bind the majority of the antibody, which gets washed away and results in a low OD, while samples with low SAM and SAH levels will leave more antibody available to bind to the conjugate, producing a high signal.,
Detection sensitivity limit of 0.2 µM SAH and 0.625 nM SAM,
Suitable for use with plasma, serum, lysates, and other biological fluid samples,
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LMO Content: None,
Host: ,
Clone: ,
Isotype: ,
Storage Temp: Upon receipt, store the SAM Conjugate, SAH Conjugate, SAM Standard and SAH Standard at -80ºC. Store the rest of the kit at 4ºC.,
LICENSE RESTRICTIONS: ,
Dangerous Goods Transportation: Excepted Quantities,
Can ship 2-day (within US only): ,
Detection Method: Colorimetric

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